LAB TRAINING MODULES

Trainees in each lab prepare short videos of relevant techniques or specialized protocols. The goal is for trainees to acquire a new skillset, where they learn how to make such videos, to share with the nucleic acid and biomaterial community. Trainees from member labs are encouraged to preview videos and engage in discussions as well as provide feedback to improve content. This will promote our program to the scientific community on a national and global level.

Please check out our NSERC Promote YouTube page to watch our latest videos!

Module 1: Flow Cytometry

This video gives a quick and basic introduction to Flow Cytometry techniques, which include cell analyzer and cell sorter. It will cover the fundamental mechanism of FACS and simple demonstration using Fortessa flow cytometer analyzer.

Credits: Molly Shoichet Lab
University of Toronto, Ontario, Canada


Module 2: Basic Introduction to DNA Synthesis

This video gives an overview of DNA synthesis that includes DNA synthesis, DNA purification and DNA quantification. It will briefly cover the main steps and will show how to synthesize DNA chemically.

Credits: Alexis Vallée-Belisle Lab
University of Montréal, Montréal, Canada


Module 3: Circular Dichroism Tutorial

This video provides helpful information on how and when to use circular dichroism (CD). CD is a very useful technique for defining the conformations of carbohydrates, proteins, or nucleic acids.

Credits: The Sleiman Lab
McGill University, Montréal, Canada


Module 4: How to make an agarose gel for electrophoresis

This video will get you started with tips on making and running an agarose gel to separate DNA fragments. It is a great opportunity to master the basic chemistry technique if you work with nucleic acids (DNA or RNA).

Credits: Functional Nucleic Acid Research Group
Yingfu Li Lab, McMaster University, Hamilton, Canada


Module 5: Introduction to Polyacrylamide Gel Electrophoresis (PAGE) for Oligonucleotides

This video gives a brief overview of Polyacrylamide gel electrophoresis using Mini-PROTEAN Tetra System BioRad. Polyacrylamide gel electrophoresis or PAGE is a molecular technique that separates macromolecules such as proteins or nucleic acids based on their size by applying an electric field.

Credits: Wilds Research Group
Concordia University, Montréal, Canada


Module 6: Detailed Course in PAGE Setup

This video will show you everything you need to set up a Polyacrylamide gel electrophoresis. The main steps are fully discussed and presented there. You can learn some tips and make your gel better!

Credits: Functional Nucleic Acid Research Group
Yingfu Li Lab, McMaster University, Hamilton, Canada


Module 7: Resazurin Cell Viability Assay

This video gives a quick step-by-step protocol for the Resazurin (phenoxazine dye) cell viability assay, also known as alamarBlue® Assay. It will cover the basic workflow performed in the tissue culture hood and provide the estimated time required to perform the assay.

Credits: McKeague Lab of Genomic Chemistry
McGill University, Montréal, Canada


Module 8: Lateral Flow Assays (LFAs)

A Lateral flow assay (LFA) is a paper-based platform for the detection and quantification of analytes in complex mixtures. In this video, we give a rudimentary introduction to an aptamer-based lateral flow assay. Discussed are its components, uses, mechanism and production.

Credits: The Laboratory for Aptamer Discovery and Development of Emerging Research (LADDER)
Maria DeRosa Lab, Carleton University


Module 9: PAGE Image Processing

We mastered the skills in making and running PAGE gels. However, do you know how to visualize your data? This video will explore a detailed procedure of image processing using Amersham Typhoon gel visualizer.

Credits: Functional Nucleic Acid Research Group
Yingfu Li Lab, McMaster University, Hamilton, Canada


Module 10: RNA radiolabeling experiment

In this video, we will show and explain how to radiolabel your RNA in the lab.

Credits: Functional Nucleic Acid Research Group
Yingfu Li Lab, McMaster University, Hamilton, Canada


Module 11: Molecular Pendulum - a universal platform for reagentless biosensing

This video gives a quick overview and a general workflow on the Molecular Pendulum (MP) - a nanoscale biosensing technology for universal, continuous biomarker monitoring developed in the Kelley Lab at the University of Toronto. It covers the fundamental working mechanism of MP, preparation and functionalization of the electronic chips to anchor MPs on gold nanostructured microelectrodes, electrochemical setup for the reagentless measurements, as well as the data collection strategy to demonstrate the user-friendliness of the technology.

Credits: Kelley Laboratory
University of Toronto, Ontario, Canada


Module 12: Surface Preparation for Single-Molecule Fluorescence Studies

This video gives a step-by-step protocol for PEG-Silane passivation of glass surfaces for single-molecule fluorescence studies.

Credits: The Cosa Group
McGill University, Montréal, Canada